ADVANCED ANALYTICAL TECHNIQUES A COMPREHENSIVE GUIDE FOR FOOD, NUTRACEUTICAL, AND PHARMACEUTICAL RESEARCH
A COMPREHENSIVE GUIDE BY FOOD RESEARCH LAB
INTRODUCTION WHY ADVANCED ANALYTICAL TECHNIQUES MATTER These techniques allow accurate identification, quantification and characterisation of complex materials (food, nutraceuticals, pharmaceuticals). They go beyond simple testing by using sophisticated instrumentation to deliver detailed molecular, structural and physical information. With increasing complexity of formulations & higher expectations for quality, safety and innovation, advanced analytics are essential.
WHAT ARE ADVANCED ANALYTICAL TECHNIQUES? Methods using scientific instrumentation, applying principles of chemistry, physics, biology to probe at molecular/atomic/structural level. KEY INFO PROVIDED Ingredient composition & purity
Chemical structure & molecular interactions
Physical attributes (texture, colour)
Contaminants & adulterants
Stability & shelf-life parameters
CATEGORIES OF TECHNIQUES MAJOR CATEGORIES OF ADVANCED ANALYTICAL TECHNIQUES Physical & Chemical Analysis Spectroscopic Techniques Chromatographic Techniques Mass Spectrometry Fractionation, Microscopy & Imaging Thermal Properties & Surface Analysis
KEY TECHNIQUES EXPLAINED EXAMPLES AND HOW THEY WORK Chromatography: e.g., HPLC for vitamins/polyphenols; GC for
flavours/pesticides; LC-MS/GC-MS for very complex mixtures. Spectroscopy: UV-Vis, FTIR, NMR, Raman – for structure, fingerprinting,
adulteration detection. Mass Spectrometry: high sensitivity, identifies molecular ions, fragments
in complex matrices. Microscopy/Imaging: SEM, TEM, particle morphology, microstructure. Thermal Analysis: DSC, TGA – evaluate stability,melting/crystallisation
transitions, moisture/volatile content
APPLICATIONS, BENEFITS & CHALLENGES APPLICATIONS Food & Beverage: authenticity, ingredient verification, contaminants, nutritional monitoring. Nutraceuticals/Supplements: quantification of bioactives, label-claims validation, quality control. Pharmaceuticals: formulation development, impurity identification, stability testing, reverse engineering.
BENEFITS High sensitivity & specificity: comprehensive characterisation; regulatory compliance; enhanced product quality; innovation support.
CHALLENGES / LIMITATIONS Cost & complexity; sample preparation; matrix interference; time-consuming.
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